Before the start of the session, hair pull test is also conducted in the same area at least three times and washing of hair is prohibited for at least two days before the treatment. The hair pull test in conducted by grasping a bunch of hair between the thumb, index and middle finger and the hair extracted An anesthetic in each session. linear in the evaluation of the overall hair volume and growth, quality, and fullness of the hair, and pictures ccs from all different angles. This is necessary for the treatment procedure, and this hair pulls test is also necessary for the preparation of the PRP.
After such evaluation the preparation of the PRP is done by collecting at least 20 cc of fresh blood. This collection of blood is done with proper aseptic precautions taken in a minor operation theater and the blood is collected in a vacutainer containing sodium citrate solution. These tubes are then rotated in the centrifugal machine which rotates at a speed of 15oo revolutions each minute for six minutes altogether. The soft spin of the first centrifugation is done to separate the blood into three different layers according to the plasma content.
The layers get arranged accordingly with the topmost layer is the cellular plasma layer containing about forty percent of the plasma and is thereby called the platelet poor plasma or PPP. The PRP layer in the middle is called the Buffy Coat and contains around five percent of the total volume. The layer at the bottom is called the RBC layer which contains the rest of the total volume. The Buffy Coat is separated further in a test tube with the help of a Finn pipette and is sent for another round of centrifugation called hard spin as it is faster at 1500 RPM and done for a longer period of 15 minutes.
The PRP settles at the bottom, and the upper layer of PPP is discarded. 9 part of this PRP is now collected in the insulin syringe which contains one part of calcium chloride which would become the activator. An anesthetic cream is applied over the bald area, cleaned with and antiseptic, and the PRP is injected using Nappage technique in which multiple injections are made each 1 cm apart in a linear pattern. With 2 to 3 cc injected every week for four weeks, you can see the result for yourself.